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. 2016 Jul 12;5(11):1473–1484. doi: 10.5966/sctm.2015-0343

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Figure 2. — Mesenchymal differentiation capacities of CD34⁺ amnion membrane-derived stem/progenitor cells and CD34⁻ amnion membrane-derived stromal fibroblast cells. After 14 days’ induction in respective differentiation medium, histochemical staining and quantitative reverse transcription-polymerase chain reaction (RT-qPCR) were performed to confirm the differentiation and quantify the relative gene expressions representative to each lineage. (A): Adipogenic: intracelluar oil droplets were seen under Oil Red O staining; osteogenic: calcified extracellular matrix was observed by Von Kossa staining; chondrogenic: chondrosphere was formed and photographed. (B): Relative expression of adipogenic, osteogenic, and chondrogenic lineage-specific genes by RT-qPCR. n = 3; ∗, p < .05. Abbreviations: AP, alkaline phosphatase; BM-MSC, bone marrow mesenchymal stromal cells; cbfA, core binding factor-α; CD34⁺, CD34⁺ amnion membrane-derived stem/progenitor cells; CD34⁻, CD34⁻ amnion membrane-derived stromal fibroblast cells.