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. 2016 Jul 12;5(11):1473–1484. doi: 10.5966/sctm.2015-0343

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Figure 3. — Comparison of multidirectional differentiation capacities between CD34⁺ AMSPCs and CD34⁻ amnion membrane-derived stromal fibroblast cells. Immunohistostaining analysis was done to detect markers for cell types from three germ layers. TuJ1: marker for neuron differentiation (ectoderm); troponin T and major histocompatibility complex: markers for cardiomyogenic differentiation (mesoderm); secretory albumin and cytokeratin 18: markers for hepatic differentiation (endoderm). Integrated morphometry analysis was done to quantify the fluorescence intensity. Scale bar = 100 μm. ∗, p < .05. Abbreviations: AMSPCs, amnion membrane-derived stem/progenitor cells; CD34⁺, CD34⁺ amnion membrane-derived stem/progenitor cells; CD34⁻, CD34⁻ amnion membrane-derived stromal fibroblast cells; DAPI, 4′,6-diamidino-2-phenylindole; GFAP, glial fibrillary acidic protein; MHC, major histocompatibility complex; TUJ1, neuron-specific class III β tubulin.