Figure 5.

Electron microscopy of implanted human iPSC-derived OPCs in EAE marmosets. (A): Electron micrograph analysis of implanted hiPSC-derived OPCs (red arrows) in EAE marmoset brain based on DAB-intensified electron-dense GFP staining. (B): DAB-intensified electron-dense GFP-labeled oligodendrocytes myelinate multiple axons in the EAE lesion in the marmoset brain (blue, nucleus; green, cytoplasm). Scale bars: 500 nm. Abbreviations: DAB, 3,3′-diaminobenzidine; EAE, experimental autoimmune encephalomyelitis; GFP, green fluorescent protein; hiPSC, human induced pluripotent stem cell; OPC, oligodendrocyte precursor cell; +Ve, positive; –Ve, negative.