Figure 5.

Ranking of overall performance of 15 iPSC-RPE preparations. mRNA and miRNA expression were normalized to hfRPE and ranked by their enrichment profiles. Functional assays were quantitatively scored relative to the physiological responses obtained from hfRPE. A nominal scale (1–5) was used to evaluate the iPSC-RPE lines, where 5 (denoted red) most closely resembles hfRPE, whereas 1 (denoted blue) bears least resemblance. Brown indicates that the data are not available. The heat map provides a graphical summary of the variability implicit in iPSC-derived RPE. iPSC-RPE with collectively low scores (lower than 3 subpar performing categories, denoted in blue) indicate inadequate attainment of the RPE phenotype (e.g., fC-iPSC-RPE3-1 and aR-iPSC-RPE5). Taking all the criteria into consideration, 20% have an overall subpar assigned ranking (less than 3, denoted blue). Abbreviations: hfRPE, human fetal retinal pigment epithelium; iPSC, induced pluripotent stem cell; RPE, retinal pigment epithelium.