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A list of the qPCR primers used to follow expression of intestinal mucin genes.
| Gene name | Accession number | Primers | Tm (°C) |
|---|---|---|---|
| MUC1 | XM_001926883.1 | F: TTAAGTGCTGTGAGCGCAAC | 59.5 |
| R: TCTGGTAGGGCTGATGGTCA | 60.0 | ||
| MUC2 | AK231524.1 | F: AGGAAATGCATCCCTCGCAA | 60.0 |
| R: GGAGCAGTCGTTCATGGTCA | 60.0 | ||
| MUC4 | XM_005670136.2 | F: TTTCTGGAGCCATGAGGGGG | 61.6 |
| R: GTCATAGTGTTTCCACCCAGGAC | 60.9 | ||
| MUC5ac | AF054584.1 | F: CGTAGAGCACAGGTGCAAGT | 59.0 |
| R: GCAGGGTCACGTTTCTCAG | 59.0 |
