Figure 1. O-GlcNAcylation of proteins secreted from normal epithelial colon cell and three different CRC cells. A: A representative SYPRO Ruby stained protein gel and B: a representative Western blot of O-GlcNAcylated proteins from cell lysates cultured in serum free-medium (SF-cell lysates) and their secreted proteins collected from conditioned medium (CM). C and D: A representative western blot of O-GlcNAcylated proteins from the secreted proteins treated, or not, with O-GlcNCAase, on-blot β-Elimination and with PNGase F, respectively. WB, Western blot.
