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Characterization of electrospun fibrous scaffolds by confocal microscope (A, C) and scanning electron microscope (SEM, B, D). Scaffolds were stained with CellTracker™ Red or sputter-coated with platinum prior to confocal and SEM imaging, respectively. Fiber diameter (E, F) was determined from SEM images using DiameterJ’s histogram analysis. Fibrous scaffolds with an average diameter of 0.5 (A, B, E) and 5 μm (C, D, F) are fabricated employing different electrospinning parameters.
