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. Author manuscript; available in PMC: 2016 Oct 19.
Published in final edited form as: ACS Appl Mater Interfaces. 2016 Jul 5;8(28):17915–17926. doi: 10.1021/acsami.6b05646

Fig. 4.

Fig. 4

Characterization of the initial attachment and spreading of MDCK cells on small and large diameter fibers, with or without RGD conjugation. (A): Confocal images of MDCK cells cultured on PCL-based scaffolds (40x). Red: F-actin; Blue: nuclei. (B): Cell spreading as a function of fiber diameter and surface chemistry; (C): Cell circularity as a function of fiber diameter and surface chemistry. The circularity is defined as 4π×area/perimeter2. (D): Average number of cells attached to the scaffold per mm2 surface area. Quantification was carried out using ImageJ software based on five separate 1,024 × 1,024 μm2 confocal images. *Significantly different (p<0.05, ANOVA) from RGD conjugated scaffolds. No significant difference was observed between small and large diameter fibers (p>0.05). Error represents standard error of the mean of 3 repeats.