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Fiber diameter affects the adhesion, spreading and scattering of MDCK cells. Cells cluster together, forming a cobble stone network in the absence of HGF on 0.5 μm fibers (A). The addition of HGF induces MDCK cells to adopt a mesenchymal phenotype (B). Cells wrapped around 5 μm fibers and adopted a spread out morphology in the absence (C) and presence (D) of HGF. Solid arrows indicate cell-cell junctions and dotted arrows point to F-actin fibers.
