Fig. 4. The effect of proteasome inhibitors on cell cycle in cholesterol-induced hypertrophy. H9c2 cells were cultured in serum-free medium for 4 h and treated with MG132 (5, 50 nM), Bortezomib (50, 500 nM), ET-1 (100 nM), and cholesterol (5 μg/ml) for 24 h. Cells were fixed and then stained for 10 min with propidium iodide to visualize the nuclei. Data were acquired on FACSCaliber and the cell cycle was analyzed using CellQuestTM of FACSCalibur Software. *P ＜ 0.05 vs control; **P ＜ 0.01 vs control.
