Fig. 2. Multimerization of ILz(6):TRAIL. (A) Recombinant ILz(6):TRAIL and recombinant TRAIL proteins were crosslinked with BMH and subjected to SDS-PAGE followed by staining with Coomassie Brilliant Blue. Monomers, dimers, and trimers of TRAIL and ILz(6):TRAIL are indicated; (B) Size fractionation of ILz(6):TRAIL. Recombinant ILz(6): TRAIL was subjected to size exclusion chromatography and the indicated relative molecular weights are comparable to molecular weight standards of dextran. (C) Multimeric form of Lz(6):TRAIL was obtained by size exclusion chromatography as described above. Sedimentation velocity AUC was performed, and AUC data were analyzed by continuous size distribution analysis using SEDFIT software (available at http://www.analyticalultracentrifugation.com).