Fig. 4. ILz(6):TRAIL is a potent killer. (A) Jurkat cells were treated with trimers, hexamers, or multimers of recombinant ILz(6):TRAIL protein for indicated times and subjected to Hoechst staining. Cells were then imaged with a fluorescent or a DIC microscope. (B) Jurkat cells were treated with trimers, hexamers, or multimers (MT) of recombinant ILz(6):TRAIL protein for 30, 60, and 120 minutes. Cell lysates were then subjected to immunoblotting against caspase-8, caspase-3, Bid, and GAPDH.
