Fig. 2. Down-regulation of genes in the p53-dependent apoptosis pathway after NPY treatment. (A) Representative immunofluorescence images of kidneys showing apoptosis (TUNEL-positive nuclei, scale bar: 70 μm). (B) Quantitative real-time PCR analysis showing the expression levels of pro- or anti-apoptotic genes in the renal tissues of each group (n = 5). (C) Western blot analysis and quantification of p53, Bax, and Bcl2 in the renal tissues of each group (n = 6). *P ＜ 0.05. All error bars indicating S.E.M. All expression levels were normalized against GAPDH mRNA expression.
