Fig. 3. NPY ameliorates cisplatin-induced renal injury through Y1 receptor signaling. (A) Expression levels of Y receptors in the kidney (n = 5), (B) BUN level, (C) Serum creatinine level (n = 5), (D) Kidney sections were stained with H&E (n = 5). scale bar: 20 μm; (E) Apoptosis in kidney tissues was examined by TUNEL assay. Representative images of TUNEL staining are shown (original magnification- 400X; scale bar, 70 μm). Quantification of TUNEL-positive cells in the tissues from each condition (n = 5). (F) Quantitative real-time PCR analysis for pro- or anti-apoptotic gene expression in the renal tissues of each group (n = 5). (G) Western blot analysis and quantification of p53, Bax, and Bcl2 levels in the renal tissues of each group (n = 6). *P ＜ 0.05. All error bars indicating S.E.M. All expression levels were normalized against GAPDH mRNA expression.
