Fig. 4. Effects of Tat-DJ-1 protein against cytokine-induced apoptotic signaling in RINm5F cells. The cells were treated with Tat-DJ-1 proteins (3 μM) for 1 h, and then treated with cytokines (5 ng/ml IL-1β, 10 ng/ml TNF-α, and 10 ng/ml IFN-γ), as described in Materials and Methods. The indicated protein expression levels were measured by Western blotting and the band intensity were measured by densitometer. *P ＜ 0.01, compared with cytokine-treated cells.
