Figure 3.

Neuroprotection from stroke is lost with deprivation of induced sleep two days after preconditioning. (A) Experimental timeline for induction of remote preconditioning, induced wakefulness (IW), and modeled ischemic stroke via middle cerebral artery occlusion (MCAO). (B) Representative coronal sections showing the absence of 2,3,5-triphenyltetrazolium hydrochloride staining in infarcted tissue (white area) 24 h after modeled ischemic stroke in preconditioned and sham (anesthetized) mice subjected to IW or left undisturbed. (C) Means ± SEM infarcted tissue in preconditioned and sham (anesthetized) mice subjected to IW or left undisturbed. **P < 0.05, two-way ANOVA with Dunn-Sidak multiple comparison. n = 6 per treatment.