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. 2016 Oct 19;12(10):e1005900. doi: 10.1371/journal.ppat.1005900

Fig 4. Knockdown of JAG1 or Notch1 up-regulates lytic gene expression.

Fig 4

(A, C) Quantification of the expression of the indicated lytic genes in iSLK.RGB cells transfected with siRNA (100 pM per well) against JAG1 (siJAG1) or scramble controls (siCtrl) in 6-well plates for 8 h when cells reached 60% confluence and treated with (A) or without doxycycline (C) for another 24 h. (B, D) Quantification of the expression of the indicated lytic genes in iSLK.RGB cells transfected with siRNA (100 pM per well) against Notch1 (siNotch1) or scramble controls (siCtrl) and treated with (B) or without doxycycline (D). Values represent the mean ± s.e.m., n = 3, *p<0.05, **p<0.01, ***p<0.001. (E–H) The efficiency of JAG1 or Notch1 depletion was detected by western blotting (E, F) and qPCR (G, H).