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. 2016 Oct 19;12(10):e1005900. doi: 10.1371/journal.ppat.1005900

Fig 6. Hes1 inhibits RTA-induced activation of KSHV lytic genes.

Fig 6

(A) HEK293T cells were plated into 12-well plates at 0.5 million cells per well, a dual luciferase assay was performed in HEK293T cells transiently transfected with expression vectors containing RTA (1 μg), various reporter plasmids containing lytic gene promoters (100 ng), and increasing amounts of Hes1 (250 ng, 500ng or 1 μg) using Lipofectamine 2000. Total transfected DNA was normalized with pcDNA3.1. (B, C) A dual luciferase assay was performed in HEK293T cells transiently transfected with different vector combinations. (B) Mutant K8 (Left) or mutant ORF59 (Right) promoters were transfected with RTA and increasing amounts of Hes1. (C) Mutant Hes1 or wild type Hes1 containing plasmids were transfected with RTA and K8 or ORF59 promoters. Data were expressed as the mean ± s.e.m., n = 3, *p<0.05, **p<0.01, ***p<0.001.