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. 2016 Oct 19;11(10):e0164837. doi: 10.1371/journal.pone.0164837

Fig 1. Quality properties of the NHC vaccine.

Fig 1

(A) The NHC protein was analyzed by SDS-PAGE in combination with PageBlue Protein Staining and by Western blotting using a C. jejuni 81116 FlaA flagellin-specific antibody. (B-E) NF-κB luciferase activity of HeLa57A cells expressing (B) chTLR5, (C) chTLR2t2/chTLR16, (D) chTLR21 and (E) chTLR4, after stimulation (5 h) with NHC flagellin or the respective positive controls. Results are expressed as fold increase in stimulated cells versus non-stimulated cells and represent the mean ± SEM of three independent experiments.