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. 2016 Oct 19;11(10):e0162821. doi: 10.1371/journal.pone.0162821

Fig 5. TUNEL staining of Capan-1 cells following a 48 h treatment with HMNE3.

Fig 5

Logarithmically growing cells were treated with the indicated doses of HMNE3 for 48 h, and the apoptotic cells were examined using the TUNEL assay. Cells from at least five randomly chosen areas on each slide were visualized and photographed using a fluorescent microscope. The percentage of apoptotic cells was determined by counting the number of apoptotic cells and dividing this value by the total number of cells in the areas.