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. 2016 Oct 19;11(10):e0164087. doi: 10.1371/journal.pone.0164087

Fig 1. Assessment of expression levels of nuclear receptor PXR, CAR and RXR-α in DEN-induced hepatic cancer.

Fig 1

A. Real-time PCR analysis was performed for PXR, CAR and RXR-α genes with the total RNA extracted from mouse liver tissues of saline-treated control and DEN-induced hepatic cancer mice. GAPDH served as an internal control. B. Cell lysate of control and hepatic cancerous mice liver tissues were electrophoresed (50 μg per sample) on a 10% SDS-PAGE. The proteins were transferred onto the methanol-activated PVDF membrane and probed with anti-mouse PXR antibody, anti-mouse CAR antibody and anti-mouse RXR-α antibody. β-Actin antibody served as control; C. The relative endogenous protein expression levels of PXR, CAR and RXR-α in control and DEN-induced hepatic cancer were quantified by densitometry. The experiments were performed with six samples of each control and DEN-induced cancerous mice. The values are represented the mean ±SE. The P-value represents the significance in DEN-induced hepatic cancer mice as compared to the control mice. P-value <0.05 represented by a single asterisk (*), P-value <0.005 represented by a double asterisk (**), P-value <0.0005 represented by a triple asterisk (***).