Fig 9. Acetyl-CoA steady-state levels were reduced in the TIGR4 spxB mutant.

TIGR4 capsule consists of several sugars that are acetylated, unlike the capsule of D39. Capsule units are abbreviated: Pyr (pyruvate); Gal (galactose); ManNAc (N-acetyl-mannosamine); FucNAc (N-acetyl-fucosamine); GalNAc (N-acetyl-galactosamine); GlcA (glucuronic acid); Glc (glucose); Rha (rhamnose) (A). Acetyl-CoA levels were measured using mass spectrometry in wild type and the spxB, lctO, and double spxB-lctO mutants in the TIGR4 (B) and D39 (C) backgrounds. Acetyl-CoA values were normalized to total cellular protein and then plotted as pmol/ mg protein. Mutants were compared to the wild type using unpaired parametric t test; *** p<0.001.