Fig 11. Loss of capsule in spxB mutant is conserved and secondary mutations can reverse this phenotype.

Capsule production was measured using capsule blotting method. Strains used include wild type, spxB mutant, and spxB mutant revertant (rev) from four strains with serotype four capsule, including TIGR4, ABCA69, ABCB20, and ABCB54 (A). Capsule production was determined for spxB deletion in strains with capsules that contained different number of acetylated sugars, including 12F and the revertant and 45 (3 acetylated sugars), 19F and 35B (1 acetylated sugar), and 6B and 18C (0 acetylated sugars) (B).