Figure 3.

The hGUF1^A609S variant exhibits increased activity in some suboptimal environments. The indicated strains were grown in full medium containing galactose to log phase. Serial 10-fold dilutions were spotted on YP plates containing 2% glucose, 0.5% glycerol or 0.5% glycerol supplemented with potassium tellurite, magnesium or chloramphenicol at the indicated concentrations and plates were incubated at 30 or 37 °C. The decreased ability of hGUF1 to complement the respiratory growth of the oxa1ΔC Δguf1 double mutant on a non-fermentable carbon source supplemented with a high concentration of magnesium or with potassium tellurite compared with hGUF1^A609S is indicated by arrows.