Skip to main content
. 2015 Oct 28;24(7):985–991. doi: 10.1038/ejhg.2015.232

Figure 4.

Figure 4

qRT-PCR screening of a molecularly unsolved PPCD cohort for ZEB1 CNV. ZEB1/TWIST1 ratio ±standard deviation is shown. (a) ZEB1 primers targeted to the 5‘ end of the gene (intron 1). Positive and negative controls include samples that were also analysed by SNP array and an unaffected (U) control DNA sample. Positive control (patient sample C17 with a confirmed deletion of the 5‘ end of the gene) and test sample for patient B8 have a ZEB1/TWIST1 ratio of ~0.5, equivalent to one copy of ZEB1. (b) ZEB1 primers targeted to the 3' end of the gene (intron 8). Positive controls (patient samples C17 and C27) and test sample for patient B8 have a ZEB1/TWIST1 ratio of ~0.5, equivalent to one copy of ZEB1.