Table 1.
Primer | Sequence | Melting temperature |
---|---|---|
HER2 forward | 5′-CAG CAG AGG ATG GAA CAC AG-3′ | 59.4 |
HER2 reverse | 5′-ACT CCT GGA TAT TGG CAC TG-3′ | 57.3 |
MUC4 forward | 5′-CTG TGT CTC TGC CTC CTT CC-3′ | 61.4 |
MUC4 reverse | 5′-TTG TTG AGC CTG TTG AGG TG-3′ | 57.3 |
β-actin forward | 5′-GGA CTT CGA GCA AGA GAT GG-3′ | 59.4 |
β-actin reverse | 5′-AGG AAG GAA GGC TGG AAG AG-3′ | 59.4 |