Fig 2. Analysis of paenilarvin as putative virulence factor during pathogenesis.
(A) At the age of 12 hours after egg hatching, honey bee larvae were infected with P. larvae DSM25430 wt and DSM25430 Δitu. Daily mortality due to P. larvae infection was recorded and total mortality after 15 days was determined. Bars represent the percentage of exposed larvae that died from American Foulbrood after 15 days. Bars represent mean values ± SD of three independent exposure bioassays for each group and 30 larvae per group. No significant difference in total mortality (Student´s t-test; p-value = 0.89) was observed. (B) Daily mortality due to P. larvae infection was recorded and cumulative mortality per day was calculated. Curves represent mean cumulative mortality ± SD for each day of 3 replicates with 30 larvae each. Analysis of the data by two-way ANOVA did not reveal any significant difference between the two curves (p-value = 0.15).