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. 2016 Oct 20;1(17):e90045. doi: 10.1172/jci.insight.90045

Figure 4. Abnormal phenotype of at-risk individual PBMCs.

Figure 4

PBMCs from control donors (C) and at-risk individuals (ARIs) (14–17 per group) were stimulated with anti-CD3 for 24 hours. The expression of indicated cytokines was quantified with ELISA or real-time PCR (A). The levels of cytokines from ARIs who are ACPA+ and ARIs who are ACPA– are compared in B. In addition, the transcript levels of PFKFB3, ATM, and G6PD measured with real-time PCR are shown in C, and the concentration of lactate in supernatant is shown in D. The transcript levels of PFKFB3 and ATM in ACPA+ and ACPA– ARIs are compared in E. Statistical analyses were performed with Student’s 2-tailed t test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. The bars shown represent mean ± SD.