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. 2016 Oct 20;1(17):e90045. doi: 10.1172/jci.insight.90045

Figure 5. Phosphatase and nonphosphatase activities of PTPN22.

Figure 5

(A) The levels of cytokines and genes in PBMCs shown in Figure 4 were plotted against the fold induction of PTPN22 shown in the right panel of Figure 3F. Statistical analysis was performed with Spearman’s correlation test. (B and C) PBMCs from at-risk individuals were transfected with plasmid vector expressing PTPN22, W620-PTPN22, CD-PTPN22, or the empty vector (–), and then stimulated with anti-CD3 for 24 hours. The levels of PTPN22, citrullinated histone H3 (cit-H3), and total histone H3 (H3) in transfected/stimulated cells were quantified by Western blotting (B). Representative blots of 6 independent experiments are shown. The density of PTPN22 and cit-H3 was quantified with densitometry and normalized against that of H3. The normalized levels of PTPN22 and cit-H3 are shown. The expression of indicated cytokines and genes was measured with ELISA or real-time PCR (C). The concentration of lactate in supernatant was quantified with a colorimetric assay (C). The data values from the same donors were connected with lines. Statistical analysis for B and C was performed with 1-way ANOVA followed by multiple comparison tests using the empty vector–transfected groups as controls. *P < 0.05; **P < 0.01; ***P < 0.001.