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Periodic acid–Schiff (PAS) staining of kidney sections (A and B) and immunofluorescent staining using polyclonal antibody against WT1 (green, C and D) in a 17.5-day embryonic kidney treated with 5 μM Nutlin-3a or DMSO for 5 days. Representative from n = 3/group. Magnification, ×10.
