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. 2016 Oct 20;1(17):e86330. doi: 10.1172/jci.insight.86330

Figure 1. Generation and characterization of Plekhm1 germline and conditional deletion mice.

Figure 1

(A) Strategy to generate a Plekhm1-flox allele. (B) Breeding strategy to generate Plekhm1 wild-type, germline deletion (KO), control (con), and conditional deletion (cKO) mice using Plekhm1-floxed mice and respective Cre-expressing mice. Hprt, hypoxanthine guanine phosphoribosyl transferase; Ctsk, cathepsin K. (C) Quantitative real-time PCR of exon 3 of murine Plekhm1 genomic DNA, normalized to the transferrin receptor 1 locus, using genomic DNA isolated from the indicated soft tissues and tibia (mean ± SD, n = 3). **P < 0.01 versus wild type by Student’s t test. (D) H&E staining of paraffin-embedded tissue sections of wild-type and Plekhm1 germline deletion mice. Representative images of each tissue from 3 different mice/genotype are shown. Original magnification: ×100. (E and F) Scatter dot plot presentations of body weight of 2-month-old male (WT, n = 17; KO, n = 17; con, n = 15; cKO, n = 12) and female mice (WT, n = 11; KO, n = 15; con, n = 14; cKO, n = 14). The mean and SD of each group are overlaid onto each column of dots.