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. 2016 Oct 20;1(17):e86330. doi: 10.1172/jci.insight.86330

Figure 5. PLEKHM1 regulates lysosome peripheral distribution and ruffled border formation in osteoclasts.

Figure 5

(A) An illustration of the polarized structures of an active osteoclast cultured on bone. The levels (upper level and bone surface level) of two representative sections of confocal microscopic images shown in B and C are indicated. (B) Immunofluorescent staining of LAMP-2 and (C) immunofluorescent staining of cathepsin K (CTSK) in wild-type and Plekhm1–/– (KO) osteoclasts cultured on glass coverslips and bone slices. White arrows in top rows point out the distribution of LAMP-2–positive lysosomes and CTSK at the periphery of WT osteoclasts. White arrowheads in the bottom rows point out the localization of LAMP-2 at the ruffled border and secretion of CTSK in the resorption lacuna inside actin rings of WT osteoclasts cultured on bone. Each image is a representative of 6 coverslips or bone slices/group/culture from at least 3 independent cultures from different mice. Scale bar: 20 μm. (D) Electron microscopic images of WT and KO osteoclasts cultured on bone slices in vitro. Images are representatives of 6 osteoclasts/group. SZ, sealing zone; RB, ruffled border. Black arrows in bottom panel point out enlarged vesicles accumulated in KO osteoclasts. Scale bar: 5 μm.