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. 2016 Oct 20;1(17):e86330. doi: 10.1172/jci.insight.86330

Figure 7. DEF8 and PLEKHM1 interact and promote each other’s binding to RAB7.

Figure 7

(A) Co-IP of HA-tagged PLEKHM1 and FLAG-tagged DEF8, KIFc1, CLASP2, and TPX2 in 293-T cells. The lysate of empty vector expressing 293-T cell (lane 1) served as a negative control. TCL, total cell lysate. (B) The structural domains of murine PLEKHM1 and DEF8. The numbers indicate the positions of amino acids. (C and D) Co-IPs of MYC-tagged DEF8, FLAG-tagged RAB7, and HA-tagged PLEKHM1 or PLEKHM1 with Y949A/L950A double mutation in 293-T cells. The experiments were repeated 3 times.