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Co-IP of HA-tagged full-length PLEKHM1 (A) or the N-terminal and the C-terminal portions of PLEKHM1 (B) with FLAG-tagged FAM98A, NDEL1, TAK1, and RAP1B in 293-T cells. The lysate of empty vector expressing 293-T cells (lane 1) served as a negative control. TCL, total cell lysate. Actin served as loading controls. The experiments were repeated 4 times.
