Figure 3.

Abnormal p62 accumulation and enhanced TLR-2/6 signaling in AP1S3-deficient keratinocytes. (a) p62 levels were measured in patient and control subject keratinocytes by Western blotting and densitometry. (b) After the transfection of silencing (AP1S3 siRNA) and nonsilencing (control) siRNA pools into primary keratinocytes, (c) baseline p62 levels were measured by Western blotting and densitometry. (d) Alternatively, cells were stimulated with MALP-2 in triplicate, and the induction of TLR2/6-dependent genes was measured by real-time PCR. The data are representative of results obtained in at least two independent experiments and are presented as mean ± standard error of the mean of duplicate stimulations. ^∗P ≤ 0.05, ^∗∗P ≤ 0.01, ^∗∗∗P ≤ 0.001. c, control; ns, not significant; siRNA, small interfering RNA; TLR, Toll-like receptor.