LIPUS regulates chondrocyte metabolism (n=3). A) IL-1β significantly increases MMP3 and ADAMTS5 mRNA expression in C-28/I2 cells. LIPUS stimulation significantly reduces IL-1β induced MMP13 and ADAMTS5 mRNA expression. B) LIPUS significantly increase Col II, aggrecan and COMP expression in chondrocytes and protects against IL-1 stimulation intensified collagen staining and represents significant difference between Ctrl and IL-1β, p=0.028, # significant difference between IL-1 and IL-1β, p=0.028, # LIPU and †nsignificant difference between Ctrl and LIPUS groups, p=0.028. Bars indicate group means and error bars show 95% confidence intervals.