Table 1.

Final HiFSA-based ¹H chemical shifts (δ_H, in ppm) and multiplicities of ecumicin (1)

Amino acid	Position	δH (ppm)	Multiplicity	Amino acid	Position	δH (ppm)	Multiplicity
N,N-Me2-l-V	2	2.672	d	l-V3	2	4.594	d
	3a	2.042	dqq		3a	2.027	dqq
	4	0.847	d		4	0.915	d
	5	0.978	d		5	0.859	d
	6,7 (N,N-Me2)	2.313	s
				N-Me-l-V	2	3.065	d
l-V1	2	4.669	d		3a	2.583	dqq
	3a	2.101	dqq		4	1.094	d
	4	0.992	d		5	0.976	d
	5	1.055	d		6 (N-Me)	3.140	s
N-Me-l-allo-I	2	4.918	d	N-Me-4-OMe-l-W	2	4.102	d
	3a	1.957	dddq		3a	3.545	dd
	4aa	0.989	ddq		3b	3.694	dd
	4ba	1.246	ddq		5b	6.698	d
	5	0.754	dd		7c	6.918	ddd
	6	0.744	d		8	6.984	dd
	7 (N-Me)	3.234	s		9	6.442	dd
					12 (O-Me)	3.826	s
l-T	2	5.167	d		13 (N-Me)	2.157	s
	3	5.783	dq
	4	1.313	d	l-V4	2	4.529	d
					3a	2.200	dqq
N-Me-l-T	2	5.018	d		4	1.028	d
	3	4.457	dq		5	0.989	d
	4	0.912	d
	5 (N-Me)	3.330	s	Ph-l-threo-S	2	4.854	d
					3	5.344	d
l-V2	2	4.842	d		5 (o)b	7.240	dddd
	3a	2.350	dqq		6 (m)b	7.255	dddd
	4	1.092	d		7 (p)b	7.201	dddd
	5	0.979	d		8 (m)b	7.255	dddd
					9 (o)b	7.240	dddd
N-Me-l-L
	2	5.110	d
	3aa	1.453	ddd	l-V5	2	4.396	d
	3ba	1.239	ddd		3a	1.968	dqq
	4a	0.957	ddqq		4	0.935	d
	5	0.168	d		5	0.919	d
	6	0.331	d
	7 (N-Me)	3.259	s

^aDefined multiplicities are assigned to signals previously reported as multiplets (m).^[1]

^bDefined multiplicities are assigned to signals previously reported as singlets (s) or broad singlets (br s).^[1]

^cHiFSA reveals the signal previously assigned as a doublet of doublets (dd)^[1] to be a ddd.