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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Endocrine. 2016 Aug 8;54(1):217–224. doi: 10.1007/s12020-016-1065-8

Fig. 1. HCG increases progesterone synthesis related gene expression in a time-dependent manner.

Fig. 1

Human granulosa lutein cells were incubated with hCG (50 ng/ml) for 0, 3, or 6 hours. Cells were then harvested at different time periods for total RNA extraction followed by reverse transcription. The real-time PCR reactions were carried out using the diluted cDNA in triplicate with predesigned primers and probes for human STAR, CYP11A1 and HSD3B1, using 18S RNA as a control. *P<.05 vs. control.