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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Sep 3;1864(12):1667–1677. doi: 10.1016/j.bbapap.2016.08.019

Fig. 2. Modulation of conformational dynamics of FXR LBD by prenylflavonoids.

Fig. 2

Time course of percent deuterium incorporation observed for apo FXR LBD in comparison to the time courses of deuterium uptake observed for co-incubation and labelling experiment of FXR LBD in presence of each of the prenylflavonoids, XN, IX, 8PN, and TX. Deuterium uptake is color-coded as indicated and mapped onto PDB 1OT7. Prenylflavonoid interactions result in gain in conformational stability in FXR-LBD reflected in lower deuterium uptake over time. Deuterium exchange data (%D) for all peptides monitored for all labeling times for FXR-LBD in presence of the different prenylflavonoids are compiled in Fig. S5. Because the N-terminal region (201–241) is not visible in the crystal structure PDB 1OT7 no color-coded HDX-MS map could be provided for this region.