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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Sep 3;1864(12):1667–1677. doi: 10.1016/j.bbapap.2016.08.019

Fig. 3. Comparison of the HDX protection profiles at the peptide level for FXR-LBD in presence of XN, IX, 8-PN and TX vs. apoprotein.

Fig. 3

Left: Deuterium exchange-in percentage difference between apo and holo FXR-LBD is shown at different reaction time points (0.5, 1, 2, 5, 10, 30, and 60 min, from left to right) for each peptide. Graphical presentations were derived from data compiled in Table S1. Deuterium level differences of regions with p = <0.001, calculated by two-way ANOVA, were considered as statistically significant and these regions are marked with an asterisk. The error bars represent the average relative standard deviation of three independently conducted labeling experiments. Right: Average differences in deuterium percentage (D%) of seven time points (0.5, 1, 2, 5, 10, 30, and 60 min) mapped onto the cartoon representation of FXR-LBD-ligand complex, PDB 1OT7. NS, “not significant” and ND, “not detected”. Due to the absence of the N-terminal region (201–241) in this crystal structure no color-coding for the HDX data could be provided for this region.