. Author manuscript; available in PMC: 2017 Dec 1.

Published in final edited form as: Biochim Biophys Acta. 2016 Sep 3;1864(12):1667–1677. doi: 10.1016/j.bbapap.2016.08.019

Table 1. Stern-Volmer constant K_SV, the fraction of fluorophore accessible to the quencher (f_á), bimolecular quenching constants (k_q), dynamic quenching constant (K_D), static quenching constant (K_S), and dissociation constant (K_d) of FXR-LBD-ligand systems studied.

These values were obtained from Eq. (S1) for IX and 8-PN, Eq. (S3) for GG (marked with *), Eq. (S4) for XN (marked with **), and Eq. (S2) and (S5) for all ligands (For details, see supplementary data Text S1).

	K_SV × 10⁵ (M⁻¹)	f_a	K_S × 10⁵ (M⁻¹)	k_q × 10¹³ (M⁻¹S⁻¹)	K_d (μM)
GG*	2.53±0.13	0.53±0.02	-	8.43±0.43	5.40±0.21
XN**	0.006±0.003 (K_D)	-	3.25±0.32	0.02±0.01	2.68±0.11
IX	1.15±0.02	-	-	3.83±0.07	5.92±0.25
8-PN	1.94±0.05	-	-	6.47±0.17	2.20±0.05

Table 1. Stern-Volmer constant KSV, the fraction of fluorophore accessible to the quencher (fá), bimolecular quenching constants (kq), dynamic quenching constant (KD), static quenching constant (KS), and dissociation constant (Kd) of FXR-LBD-ligand systems studied.