Table 2.
Kinetic data for the four hybrid enzymes
| dF393A-1 | dF393W-1 | sL407C-1 | dQ403W-1 | |
|---|---|---|---|---|
| reduction rate (kET) | ||||
| SFb | n.d.a | 1.20x105 ± 0.06 s−1 | 1.49x105 ± 0.02 s−1 | 2.02x105 ± 0.01s−1 |
| SB | 1.04x105 ± 0.02 s−1 | 6.25x104 ± 0.01 s−1 | 7.32x104 ± 0.04 s−1 | 1.45x105 ± 0.02s−1 |
| kcat (eq.min−1), | < 2 | 21 | 36c | 44 |
| Km (μM) | 11.3 | 5.0 | 11.0c | 9.5 |
| TTNd | <5 | 192 | 230 | 420 |
a
could not be determined as the SF-dF393A-1 was unstable under the laser experiments leading to the SB-F393A-1 species;
b
SF: Substrate free and N-palmitoylglycine bound (SB);
c
value determined previously[15]
d
TTN: total turnover numbers as nmol of products / nmol of enzymes at the end of the reaction.