Table 2.

Evaluation of antimicrobial activity^a and biofilm synthesis^b of different Bacillus spp. isolates.

Bacillus isolates	S. Enteritidis (mm)	E. coli (mm)	C. difficile (mm)	Biofilm formation
AM0902	0.0 ± 0.00	0.0 ± 0.00	0.0 ± 0.00	+
AM0904	12.0 ± 0.38*	16.0 ± 2.31	26.0 ± 1.86	+
AM0905	6.7 ± 0.67	14.0 ± 1.15	20.3 ± 1.67	++
AM0908	6.0 ± 0.56	4.3 ± 0.33	22.0 ± 2.31	+
AM0923	7.7 ± 0.30	10.0 ± 3.06	24.0 ± 3.06	+
AM0933	1.3 ± 0.33	4.0 ± 0.58	10.0 ± 1.15	++
AM0934	6.3 ± 0.40	8.7 ± 1.76	22.7 ± 2.40	+
AM0938	8.0 ± 1.15	10.0 ± 2.00	22.0 ± 2.00	+
AM0939	6.3 ± 0.88	8.3 ± 1.33	26.0 ± 2.60	+
AM0940	8.0 ± 1.12	10.3 ± 1.67	21.0 ± 1.76	++
AM0941	0.7 ± 0.27	0.0 ± 0.00	0.0 ± 0.00	++
AM1002	5.7 ± 0.58	8.7 ± 1.76	16.0 ± 2.08	++
AM1010	8.0 ± 1.10	20.0 ± 1.45*	28.0 ± 2.67*	+
AM1011	8.5 ± 0.90	10.7 ± 1.76	20.3 ± 2.33	++
AM1012	8.7 ± 0.88	20.0 ± 2.19*	10.0 ± 1.75	++
AM1013	4.0 ± 1.15	10.0 ± 1.15	22.0 ± 1.15	+
AM1109A	10.3 ± 1.20	12.0 ± 1.50	24.0 ± 1.11	++
AM1109B	0.3 ± 0.33	0.0 ± 0.00	14.7 ± 1.62	++
B2/53	10.3 ± 1.20	12.0 ± 0.58	26.0 ± 3.08	+
BL	0.0 ± 0.00	4.0 ± 0.52	10.0 ± 2.00	+
JD17	6.3 ± 0.33	10.0 ± 1.15	20.6 ± 3.53	+
JD19	2.0 ± 0.58	2.7 ± 0.67	19.0 ± 1.72	+
NP001	8.0 ± 0.88	6.0 ± 0.58	12.0 ± 1.13	+
NP002	4.3 ± 1.33	6.0 ± 1.10	20.7 ± 2.40	+
NP117B	2.7 ± 0.67	6.0 ± 1.15	18.0 ± 3.46	+
NP121	2.3 ± 0.33	14.0 ± 3.06	16.0 ± 2.31	+
NP122	13.7 ± 1.86*	12.0 ± 2.00	26.0 ± 4.16	++
NP124	6.0 ± 1.73	12.0 ± 1.86	22.0 ± 2.03	+
NP126	0.3 ± 0.30	2.0 ± 1.89	21.7 ± 1.76	+
MM65	8.0 ± 0.55	10.0 ± 1.15	20.3 ± 1.45	++
RW41	5.7 ± 0.88	10.0 ± 2.00	22.0 ± 2.28	+

*Identified bacterial strains with the enhanced antimicrobial activity, P < 0.05.

^aRepresents the diameter of the zone of inhibition observed at 24 h of incubation without the diameter of the bacterial colony. Data expressed as mean ± SE.

^bThe qualitative measurement of biofilm synthesis was based on color intensity and size of the adherent crystal violet ring with a score ranging from negative (−) to strong (++) biofilm formation. All Bacillus spp. isolates were tested by triplicate.