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. 2016 Aug 12;52(5):461–478. doi: 10.1007/s11627-016-9776-3

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Figure 1. — (a) Transgenic somatic embryos and (b) plant of cassava cv. 60444 transformed with Ensifer adaherens OV14, expressing GUS. Note the formation of nodules on roots (arrows). This event was one of the three obtained for which a Southern blot (c), confirmed the presence of single copy insertions (first and third lanes) as well as multicopies of the T-DNA (second lane; fourth lane is control transgenic plant).