Figure 2.

Phylogenetic relationship analysis of GH3 family and sequence-based grouping of plant GH3 proteins based on acyl acid binding site residues. (A) CpGH3 and AtGH3 proteins are color coded according to their subgroup. Sequences corresponding to the residues of the α5 and α6 motifs, to residues 217 and 299 of AtGH3.12, and to the β8 and β9 motifs are indicated (Westfall et al., 2012). Conserved residues are highlighted by colored boxes. (B) Analysis of IAA-amido synthetases activities of CpGH3 proteins. All five GH3 proteins were purified from E. coli, and the E. coli with empty vector was used as negative control. The activities have been normalized to μg of protein.