Figure 4.
ASNS knockdown inhibits tumor growth in vivo.
(A) Xenograft size of HCT116 cells transfected with control or shASNS vector. A total of 4 × 106 cells were subcutaneously injected into the flank of 4- to 6-week-old female nude mice, and then tumor volumes were measured 2 times per week (each group, n = 5). Representative images of tumors are shown. (B) H&E and immumohistochemical staining for ASNS, CD34, and Ki67 in xenograft tumors. Upper panels show serial sections of control tumors, whereas lower panels show those of shASNS-transfected tumors. Scale bar, 50 μm. (C) Ki67-positive cell density. Cell numbers were counted in five random fields (each group, n = 3). Mean; bars, ± SD (Student's t test; *P < .05). (D) Microvessel density. CD34-positive cells were counted in five random fields (each group, n = 3). Mean; bars, ± SD (Student's t test; NS: not significant).