Figure 4.

Loss of Twist2 increases the sensitivity of thymocytes to TCR-mediated apoptosis. (a) The percentage of AnnexinV⁺ cells in TCRβ/CD69 subpopulations as in Figure 3c is summarized (error bars, ±S.E.M., n=6). (b) The percentage of DP cells in thymocytes obtained from DO-TCR-Tg mice was detected at 24 h after co-culture with OVAp (OVA_323-339 peptide) pre-loaded B cells using FACS CantoII. The graph presents the relative percentage of DP cells to non-stimulated cells (error bars, ±S.E.M., n=6). (c) Indicated mice were crossed with Tcrα^−/− mice to preclude TCR-experienced thymocytes. Apoptotic cells were detected after PMA and IM stimulation using AnnexinV staining kit. The graph presents the AnnexinV⁺ percentage in control and cKO mice (error bars, ±S.E.M., n=3)