Figure 5.

Twist2 inhibits TCR-mediated apoptosis and negative selection of thymocytes. (a) Indicated mice were crossed with Tcrα^−/− mice. Apoptotic cells were detected after PMA and IM stimulation using AnnexinV staining kit. The graph presents the AnnexinV⁺ percentage in NLC and Twist2-Tg mice (error bars, ±S.E.M., n=3). (b) Representative CD4/CD8 and CD24/Vα2 FACS plots of the indicated mice. (c) The graph presents the total thymic cellularity in the indicated mice (left). Relative percentage of thymic cellularity in OT2-TCR × mOVA-Tg mice and OT2-TCR-Tg mice with either NLC or Twist2-Tg mice is shown (right) (error bars, ±S.E.M., n=3). (d) The percentage of the CD24^loVα2^hi population in total thymocytes from the indicated mice is summarized (error bars, ±S.E.M., n=3)