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. 2016 Oct 20;7:374. doi: 10.3389/fphar.2016.00374

Figure 2.

Figure 2

Effects of ammonium acetate and buffer pH on the migration of analytes (0.50 μg/mL each). HPLC profiles of (A) 5 mM ammonium acetate and buffer pH 3.5, (B) 4 mM ammonium acetate and buffer pH 3.5, (C) 4 mM ammonium acetate and buffer pH 2.5, (D) 4 mM ammonium acetate and buffer pH 3.0, (E) 4 mM ammonium acetate and buffer pH 4.0. Peaks: 1, resveratroloside (Res); 2, sennoside A (Se-A); 3, baicalin (Ba); 4, coptisine (Co); 5, palmatine (Pa); 6, berberine (Be); 7, rhein (Rh); 8, aloe-emodin (Ale); 9, wogonin (Wo). HPLC condition in different ammonium acetate and buffer pH values: column, Agilent Poroshell 120 HPLC column (Agilent Technologies, Inc., USA, 150 × 4.6 mm I.D., 2.7 μm); mobile phase: an acetonitrile acetic-acetate buffer (4~5 mM; H2O, pH 2.5~4), 2:98 (v/v); flow rate, 0.6 mL/min; injection volume, 10 μL; UV detection: λ: 254 nm.