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. 2016 Oct 20;7:374. doi: 10.3389/fphar.2016.00374

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Copyright © 2016 Wu, Chang, Liou, Lo, Chung, Lee, Chi, Du, Wong, Juo, Lee and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of five different gradient elution programs on the migration of analytes (0.50 μg/mL each). HPLC profiles of (A) Program I, (B) Program II, (C) Program III, (D) Program IV, (E) Program V were demonstrated.